DUBAI | 3 DAY TRAINING COURSE IN NEXT GENERATION SEQUENCING DATA ANALYSIS AND CRISPR Cas9

BioDiscovery Group, India is an expert in conducting Workshops, Research Projects, Seminars & Training on topics of Bioinformatics specially in Gene & Genome Editing by CRISPR/Cas9, NGS Data Analysis, Molecular Modeling, etc. We are carrying out several programs to make learn the technology to students/ researchers/ professionals from Biotechnology/ Bioinformatics/ Pharmacy/ Chemistry/ Veterinary background all over the world.

We are glad to announce 3 day training cum workshop on Next Generation Sequencing Data Analysis and Gene Editing by CRISPR/Cas9.

The program will be conducted for 3 days for which participants have to bring their own laptop for which the Linux environment will be provided by us so that they can use that for running training processes on their laptops. We will provide handouts (soft & hard copy both) along with the software and paper of technology. It will be extensive training program which will make the participants do all the processes on their Computer Systems (hands-on training) with complete learning of this latest technology.The course will be of great help to students/ researchers/ faculty/ scientists in learning this latest science and technology.

BRING YOUR LAPTOP HAVING WINDOWS OS WITH FOLLOWING SYSTEM CONFIGURATION

• Processor – i3 or above.
• RAM – 8 GB or above. (4 GB will be fine)
• Hard Disk – 500 GB or above.

TOPICS

• Introduction to CRISPR for Gene editing
• Mechanism of CRISPR.
• Pipeline of CRISPR cas9.
• Finding sgRNA target sites using computational tools.
• Primer designing for library preparation.
• Basic Terminologies.
• Introduction to file types in NGS.
• What is Sequence alignment?
• Introduction to Genome Analysis
• Database and File Formats in NGS.
• NCBI Genomics Library.
• NCBI resources used in Next Generation Sequencing.
• Introduction to analysis of Next Generation Sequencing Data.
• Install Ubuntu on a windows machine for Sequencing Data Analysis.
• What is the FASTQ format (Download files from NCBIs SRA)

PRACTICAL APPLICATION

Hands-on exercises will be performed individually using software tools on your own laptops. No prior software experience is required.

• Using caRpools software.
• Blasting into SRA using NCBI tools.
• Retrieving & Checking the quality of Sequence using NCBI tools.
• Check your sequenced reads for quality (How to install use FastQC) using NCBI tools.
• Process Sequence Reads-Quality trimming read joining etc. using ea-utils.
• Align Immunome Data with IMGT.
• Align reads using MIXCR.
• tcR package (R package for TCR sequencing).
• TCR Sequencing Analysis using tcR Package.
• Immunome Data Analysis using VDJ Tools.

OUTCOME- Participants will

• Learn the technology behind editing the genomes.
• Learn to look into the genome for checking the mutation.
• Be able to understand the technology behind the sequencing of human genome.
• Learn how to use immunology data for cancer treatment/research.
• Learn to retrieve and check the quality of sequences.
• Learn aligning the target sequence with reference sequence.
• Learn sequence analysis.
• Learn data analysis of our result based on different parameters.
• Learn the overall concept of Next Generation Sequencing Data Analysis.

TARGET AUDIENCE With basic knowledge in Life Science and Genomics that would like to receive a comprehensive overview and hands on experience in Next Generation Sequencing Data Analysis and CRISPR the target audience comprises: Student: Bachelor, Masters, PhD, MBBS, MD students as well as Faculty and Professors from Microbiology, Biochemistry, Biotechnology, Immunology, Medicine, Pharmacy, Pharmaceutical Chemistry, Biomedical Technology, Genetics, Bioinformatics, Plant Science and Life Sciences. Professionals: Resident Doctors, Biotechnology, Bioinformatics, Medicine and Pharmaceutical scientists from industry, academia and regulatory agencies. Hands-on exercises will be performed individually using software tools on your own laptops. (no prior experience required).